D-Luciferin sodium salt
Substrate for firefly luciferase, properties and use in assays of ATP and luciferase
AQC (6-Aminoquinolyl-N-hydroxysuccinimidyl carbamate)
6-Aminoquinolyl-N-hydroxysuccinimidyl carbamate is used to detect proteins and amino acids. It reacts with amino acids forming stable unsymmetrical urea derivatives. It can be used with HPLC analysis.
References:
https://www.ncbi.nlm.nih.gov/pubmed/16586471
http://pubs.rsc.org/en/content/articlelanding/2017/qm/c7qm00076f#!divAbstract
D-Luciferin potassium salt
Substrate for firefly luciferase, properties and use in assays of ATP and luciferase
Out of stock
Coelenterazine native
Coelenterazine is a luminophore found in aquatic organisms and is the substrate for many luciferase enzymes, including Renilla reniformis, Gaussia and aequorins. It is commonly used for bioluminescent calcium detection and the monitoring of reporter genes in BRET (Bioluminescence Resonance Energy Transfer), ELISA and HTS methods as well as chemiluminescent detection of superoxide anions and peroxynitrite in cells or tissues.
Out of stock
S-HyNic Crosslinker
The S-HyNic (succinimidyl-6-hydrazino-nicotinamide) heterobifunctional crosslinker is fundamental to Chromaling. S-HyNic reacts with primary amines of proteins (amino group of glycine) or amino-modified oligonucleotides or surfaces, introducing a HyNic (6-hydrazinonicotinamide) linker that forms stable covalent conjugates with biomolecules possessing 4FB (4-formylbenzamide) incorporated linkers.
References:
https://www.researchgate.net/scientific-contributions/39596054_M_J_Baas
D-Luciferin free acid
D-Luciferin is a common bioluminescent reporter used for in vivo imaging of the expression of luciferase. The water soluble substrate for the firefly luciferase enzyme uses ATP and Mg-Ion as cofactors to emit a characteristic yellow-green emission in the presence of oxygen, which shifts to red light in vivo at 37°C. Through the utilization of ATP, the reaction can be further used to indicate the presence of energy or life in order to function as a life-death indicator.
Luciferin is a common reagent used throughout the biotechnology family and specifically for in vivo imaging. Luciferase labeled tumor cells, stem cells or infectious diseases are often inoculated into research animals such as rats or mice for investigation. This is often made by the use of the D-Luciferin sodium salt (BC218), because of it`s good solubility in water and tolerance of living organism. The injection of luciferin allows for the real-time, noninvasive monitoring of disease progression and/or drug efficacy in these model systems through Bioluminescence Imaging (BLI).
Luciferin is also commonly used for in vitro research, including luciferase and ATP assays, gene reporter assays, high throughput sequencing and various contamination assays.
Firefly Luciferin is identical to Beetle Luciferin.
References:
https://www.frontiersin.org/journals/immunology/articles/10.3389/fimmu.2018.03097/full
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3566035/
SANH
Hydrazine-based and carbonyl-based bifunctional crosslinking reagents for biomolecules, drugs, and synthetic polymers
10H-Phenothiazine-10-propanesulfonic acid sodium salt
phenothiazine sodium salt is a water-soluble enhancer of luminol chemiluminescent peroxidation catalyzed by horseradish peroxidase (HRP). It also enhances soybean peroxidase-induced chemiluminescence, increasing sensitivity and lowering the detection limit from 1.1 to 0.18 pM
